The neuron-astrocyte synaptic complex is a fundamental unit in the central nervous system (CNS). How astrocytes become developmentally mature remains largely unknown. Astrocytes play a vital role in the synaptic transmission via the regulation of glutamate transporter GLT1 and its human homology, EAAT2. Loss of GLT1/EAAT2 is often involved in CNS disease including amyotrophic lateral sclerosis (ALS). In addition, DNA methylation of GLT1/EAAT2 promoters is involved in GLT1/EAAT2 expression regulation. My research focuses on understanding the molecular regulation of developmental maturation of astroglia, by specifically focusing on the transcriptional and post-transcriptional mechanism of GLT1/EAAT2.