The central theme of the Rosen laboratory is understanding the metabolic and biochemical fate of marrow stromal cells as progenitor osteoblasts and/or adipocytes. In vivo, this translates into defining the relationship between marrow adipogenesis and osteoblastogenesis, and the interactions between whole body and skeletal metabolism. We use age, genetic, environmental, diet, and pharmacologic manipulations in order to understand the complex regulation of bone remodeling. We use a variety of techniques to address our research questions, including DXA imaging, NMR, microCT, MRI, osmium tetroxide staining, histomorphometry, immunohistochemistry, immunofluorescence, confocal microscopy, Seahorse extracellular flux analysis, and metabolic in vivo studies using Promethion technology.